In vivo veritas - Practical Course in Animal Research [WBT-MBT2-23E] Semestr letni 2020/2021
Ćwiczenia, grupa nr 1

- Hans J Hedrich, Gillian Bullock, The Laboratory Mouse, Elsevier Ltd, 2012;

- Piper M. Treuting, Suzanne M. Dintzis, Comparative Anatomy and Histology: A Mouse and Human Atlas, Academic Press, 2012;

- John J. Bogdanske, Scott Hubbard-Van Stelle, Margaret Rankin Riley, Beth M. Schiffman, Laboratory Mouse and Laboratory Rat Procedural Techniques, CRC Press, 2010;

- Richard E. Fish, Marilyn J. Brown, Peggy J. Danneman and Alicia Z. Karas, Anesthesia and Analgesia in Laboratory Animals, American College of Laboratory Animal Medicine, 2008;

- Committee on Recognition and Alleviation of Pain in Laboratory Animals; Institute for Laboratory Animal Research; Division on Earth and Life Studies; National Research Council, Recognition and Alleviation of Pain in Laboratory Animals, National Academies Press (US), 2009,

- e-learning

Biophysics Department:

1 – The mouse as an experimental animal. The rules of the animal house. Animal welfare. Animal handling, gender recognition, behavioural observations.

2 – Tagging animals in the experiment. Intragastric, intraperitoneal, intravenous, subcutaneous and intramuscular injections.

3 – Tail vain injections. Methods of blood collection. Methods of euthanasia. Section of a mouse and anatomical analysis. Transport of laboratory animals.

4 – Mice shaving and depilation. Anaesthesia and analgesia. Health monitoring.

5 – Monitoring of the experiment (vital signs, behavioural cages, metabolic cages, imaging, etc.). Urine collection. Blood collection from the heart. Section of the mouse; organs and tissues collection. Histological preparation (tissues preparation steps, paraffin and frozen method of fixation, presentation of histological slides).

6 - Lymph nodes mapping in the mouse. Animal models used in the experiments. Special strains of laboratory animals (nude, SCID, transgenic animals). Improvement of previously known techniques.

Department of General Biochemistry:

7 - Genotyping of transgenic mice

8 - Bone marrow derived cells - isolation and culture

9 - Culture of peritoneal macrophages

10 - Culture of primary keratynocytes and fibroblasts

11 - Preparation of single cell suspention from different murine organs

e-learning

Demonstrating methods – show combined with experience

Expository methods – description

Expository methods – explanation or clarification

Expository methods – formal lecture

Expository methods – multimedia presentation

Practical methods - laboratories

Practical methods – demonstration

Practical methods – guided discovery

Problem-solving methods – discussion

Problem-solving methods – participatory methods - discussion

Problem-solving methods – participatory methods - seminar

The final evaluation is the result of the sum of points obtained during participation in the course and final test;

Evaluation:

Active participation in the course (40pkt.)

Execution of tasks on e-learning platform (20pkt.)

Written tests (40pkt.)

For a positive evaluation is necessary to obtain points from each of the above and participate in 12 from 13 classes.

Biophysics Department (50 points):

4 points - LABOLATORY NOTEBOOK and ANIMAL OBSERVATION CART

16 points - E-Learning (Pegaz platform):

Materials and protocols, Tasks (Experimental proposal, Cages enrichment, Ethical mind map, Law questions, Analysis of hairs regrowth after depilation and shaving)

20 points - EXAM

50 p. – General Biochemistry Department:

20 p. - Attendance and laboratory notebook

10 p. - Tasks

The course will take place in summer semester 2020/2021

Room 1.01.6

Hours: 10:30-14:15

First seminar: 11.10.2019

First lab: 18.10.2019